发表论文： | 一、个人简历： 宋长年，男，博士，讲师。2006 - 2011 必威，硕博连读； 2011.6 - 今 必威betway中国官方网站，讲师。 二、主要研究方向 葡萄抗逆境胁迫的基因组生物学与生产技术研发 三、研究课题： 1、miR172及其靶基因APETALA2的表达与功能分析, 2009.10-2011.7, 江苏省公司产品创新工程项目(编号CX09B_238Z)。3万，主持人。 2、枳microRNA172调控制AP2类似基因的表达和功能分析, 2009.2-2012. 2, 教育部科学技术研究重点项目 (编号: 109084)。10万，骨干，负责基因表达分析。 四、研究成果： Song Changnian, Fang Jinggui, Wang Chen, Guo Lei, Nicholas Kibet Korir, Ma Zhengqiang. 2010. miR-RACE, a new efficient approach to determine the precise sequences of computationally identified trifoliate orange (Poncirus trifoliata) microRNAs. PLOS one, 5(6): e10861. Song Changnian, Wang Chen, Zhang Changqing, Kibet Korir Nicholas, Yu Huaping, MaZhengqiang, Fang Jinggui. 2010. Deep sequencing discovery of novel and conserved microRNAs in trifoliate orange (Citrus trifoliata). BMC genomics, 11:431. Song Changnian, Fang Jinggui, Li Xiaoying, Liu Hong, Chao C. Thomas. 2009. Identification and characterization of 27 conserved microRNAs in citrus. Planta, 230, 671-685. Song Changnian, Jia Q, Fang Jinggui, Li Fei, Wang Chen, Zhang Zhen. 2010. Computational identification of citrus microRNAs and target analysis in citrus expressed sequence tags. Plant Biology, 12: 927-934. Song Changnian, Cao Xue, Fang Jinggui, Wang Chen, Nicholas Korir Kibet, Zhang Zhen. 2010. A practical protocol for extraction of low molecular weight RNA from Citrus trifolita tissues for microRNA Northern Blotting and RT-PCR. African Journal of Biolotechnology, 9: 8726-8730. Fang Jinggui, Song Changnian, Zheng Y, Chao C T. 2008. Cytosine methylation variation in elationsh cultivars. The Journal of Horticultural Sciences & Biotechnology. 83: 833-839. Fang Jinggui, Song Changnian, Qian Jianling, Zhang Xiaoyin, Shangguan Lingfei, Yu Huaping, Wang Xichen. 2010. Variation of cytosine methylation in fifty seven sweet orange cultivars. Acta Physiologiae Plantarum, 32: 1023-1030. Yu Huaping, Song Changnian, Jia Qidong, Wang Chen, Li Fei, Nicholas Korir Kibet, Zhang Xiaoyin, Fang Jinggui. 2011. Computational identification of microRNAs in apple expressed sequence tags and validation of their precise sequences by miR-RACE. Physiologia Plantarum, 141: 56-70. Wang Chen, Wang Xicheng, Nicholas Korir Kibet, Song Changnian, Zhang Changqing, Li Xiaoying, Fang Jinggui. 2011. Deep sequencing of grapevine flower and berry short RNA library for discovery of novel microRNAs and validation of precise sequences of grapevine microRNAs deposited in miRBase. Physiologia Plantarum, 143: 64-81. Li Xiaoying, Shangguan Linfei, Song Changnian, Wang Chen, Gao Zhihong, Yu Huaping, Fang Jinggui. 2010. Analysis of expressed sequence tags from Prunus mume flower and fruit and development of simple sequence repeat markers. BMC Genetics, 11: 66. Fang Jinggui, Wang Chen, Yu Huaping, Zheng Y, Li Xiaoying, Song Changnian, Chen J. 2009. Identification of 57 navel sweet orange cultivars with AFLP markers. The Journal of Horticultural Sciences & Biotechnology. 84: 585-590. Wang C, Shangguan L, Kibet KN, Wang X, Han J, Song C, Fang J. 2011. Characterization of microRNAs Identified in a Table Grapevine Cultivar with Validation of Computationally Predicted Grapevine miRNAs by miR-RACE. PLoS One. 6: e21259. Zhang Y, Yu M, Yu H, Han J, Song C, Ma R, Fang J. 2011. Computational identification of microRNAs in peach expressed sequence tags and validation of their precise sequences by miR-RACE. Mol Biol Rep, 39：1975-1987 宋长年, 房经贵, 王晨, 上官凌飞, 章镇. 2009. 基于EST库的枳APETALA2基因cDNA克隆及其表达分析. 园艺学报, 36(6): 799-806. 宋长年, 贾启东, 王晨, 李飞, 章镇, 房经贵. 2010. 32种果树的microRNA的生物信息学预测与分析. 园艺学报, 37(6): 869-879. 宋长年, 钱剑林, 房经贵, 王化坤, 邱学林, 章镇, 张晓莹. 2010. 枳SPL9和SPL13全长cDNA克隆、亚细胞定位和表达分析. 中国农业科学, 43(10): 2105-2114 王晨, 刘洪, 房经贵, 宋长年, 李晓颖, 章镇. 2010. 基于EST库的葡萄(Vitis vinifera) APETAAL2基因cDNA克隆及其表达分析. 果树学报, 27(2): 207-212.. 杨 光, 曹 雪, 房经贵, 宋长年, 陶建敏, 王 晨, 初建青. 2010. 葡萄7个重要花发育相关基因序列特征的分析. 江西农业学报, 22 (2) : 49-52. 郭磊, 韩键, 宋长年, 房经贵. 2011. 葡萄砧木研究概况. 江苏林业科技, 3: 48-54. 曹雪, 王晨, 房经贵, 杨光, 于华平, 宋长年. 2011. 葡萄SPL9和SPL10基因全长cDNA克隆、亚细胞定位和表达分析. 园艺学报, 2: 240-250. 上官凌飞, 王晨, 房经贵, 李晓颖, 王西成, 宋长年. 2011. 利用GenBank中大量葡萄EST序列分离有效基因的电子表达分析平台. 中国农业科学, 13: 2748-2759. 孙欣, 上官凌飞, 房经贵, 宋长年, 王晨, 慕茜. 2011. 葡萄NAC转录因子家族生物信息学分析. 基因组学与应用生物学, 2: 229-242. 孙欣, 李荷蓉, 宋长年, 房经贵. 2011. 第10届国际葡萄遗传与基因组学会议递交论文情况分析. 中外葡萄与葡萄酒, 3: 67-70. 冷翔鹏, 孙欣, 房经贵, 宋长年. 2012. 波尔多液及其在果树生产上的应用, 江苏农业科学, 40(2): 97-99.
五. 发明专利 梅、杏及杏梅的PCR快速鉴定区分方法(专利号ZL200810234569.9),2011.09.完成人排序:7/9 六、获得荣誉： 必威betway中国官方网站第二届感动必威betway中国官方网站人物 七、社会兼职： 江苏省细胞与发育生物学学会学术交流部委员 八、联系方式： 025-84399069 (Tel) Email：songchangnian@njau.edu.cn；songchangnian@126.com |
